失礼ですが、もう一度おっしゃって下さい。

I beg your pardon?

失礼(しつれい)ですが、もう一度(いちど)おっしゃって下(くだ)さい。

Shitsurei desu ga, mōichido osshatte kudasai.

失礼 (しつれい、しつらい、しちらい)

discourtesy; impoliteness; Excuse me; Goodbye; to leave; to be rude

です (です)

be; is

が (が)

indicates sentence subject (occasionally object); indicates possessive (esp. in literary expressions); but; however; still; and

もう一度 (もういちど)

once more; again

仰っしゃる (おっしゃる)

to say; to speak; to tell; to talk

下さる (くださる)

to give; to confer; to bestow; to kindly do for one; to oblige; to favour; to favor

Readings: シツ、うしな.う、う.せる

Meanings: lose, error, fault, disadvantage, loss

Readings: レイ、ライ

Meanings: salute, bow, ceremony, thanks, remuneration

Readings: イチ、イツ、ひと-、ひと.つ

Meanings: one, one radical (no.1)

Readings: ド、ト、タク、たび、 -た.い

Meanings: degrees, occurrence, time, counter for occurrences, consider, attitude

Readings: カ、ゲ、した、しも、もと、さ.げる、さ.がる、くだ.る、くだ.り、くだ.す、 -くだ.す、くだ.さる、お.ろす、お.りる

Meanings: below, down, descend, give, low, inferior